In vitro experiment, TNF-α alone obviously increased the NF-κBp65 subunit expression in the VSMCs compared with control group, while NF-κBp65 subunit expression was significantly down-regulated in the VSMCs in TNF-α together with zinc group(Fig 5B).The above results indicated zinc inhibited the NF-κB activation in the AAA. Here, NFKB1 is linked to triple-A syndrome.