The AUC of using ATRX loss as a diagnostic biomarker for discriminating between pGBM, oligodendroglioma (WHO grade II/III) and sGBM, astrocytoma (WHO grade II/III) was 0.8241 (sensitivity 76.67%, specificity 88.15%, Figure 3B). Here, ATRX is linked to astrocytoma (excluding glioblastoma).