Western blotting showed that selinexor slightly decreased the total protein expression of KIT and phosphorylated KIT but exhibited no effect on the phosphorylation of downstream molecules (AKT and MAPK) in GIST-T1 cells, whereas imatinib caused a dramatic decrease in phosphorylation of KIT as well as of downstream molecules (Figure 4B). Here, AKT1 is linked to gastrointestinal stromal tumor.