The prostate cancer cell lines that were chosen represent well-characterized tumor states (i.e., androgen dependent (LNCaP), androgen sensitive (LNCaP/C4-2) and castration resistant (22Rv1)), and also include examination of mutated forms of AR (i.e., LNCaP) and AR splice variants (i.e., 22Rv1). This evidence concerns the gene AR and Familial prostate cancer.