PRKCD and non-small cell lung carcinoma: To determine if decreased expression of integrin αVβ3 upon depletion of PKCδ correlates with reduced transformed growth, we assayed the colony forming ability of NSCLC cells that were detached and re-plated immediately into a clonogenic survival assay (“adherent culture”, Figure 4A), and of cells that were cultured for 24 hours on poly-HEMA coated plates to prevent adhesion, and then re-plated into a clonogenic survival assay (“suspension culture”, Figure 4C).