FN1 and melanoma: Using semiquantitative RT-PCR, we then analyzed the mRNA levels of FN1 (with primers amplifying FN1 with and without the extradomains A and B: EDA+, EDA-, EDB+, and EDB- FN1), ITGB3, and NFATC2 in WM115, WM793, WM239, MM170, SKMEL-28, SKMEL-103, SKMEL-147, and BLM melanoma cell lines.