As shown in Additional file 4: Figure S4, TA-CIN protein vaccine boost following priming with pNGVL4a-CRT-E6E7L2 DNA vaccine does not generate a higher number of TA-CIN-specific CD4+ T cells or E6-specific CD8+ T cells in mice compared to three doses of pNGVL4a-CRT-E6E7L2 DNA vaccinations or three dose of TA-CIN protein vaccinations. This evidence concerns the gene CD4 and cervical squamous intraepithelial neoplasia.