As shown in Fig. 3b and c, tumor-bearing mice that received two pNGVL4a-Sig/E7(detox)/HSP70 DNA (25 μg) priming vaccinations followed by a single boost with the higher dose of TA-CIN protein (25 μg) induced the highest percentage E7-specific CD8+ T cells regardless of administration route, which is consistent with the results observed in naïve mice (Fig. 2b–e). The gene discussed is CD8A; the disease is neoplasm.