Indeed, when a mutant TMEM16F was introduced into a mouse lymphoma cell (W3-Ildm) to achieve constitutive PS exposure, PS-positive tumor cells (assessed as annexin V positive) were not engulfed by professional DCs, and only became phagocytosed after activation of caspase 3 and Xkr8 with Fas antibody.25 Thus, the PS externalized by TMEM16 does not provide an eat-me signal, but is sufficient to provide an electrostatic charge to recruit clotting factors via the interactions of their Ca2+-dependent Gla domains. This evidence concerns the gene ANXA5 and neoplasm.