Interestingly, some p53 target genes, such as apoptosis mediators BAX, PUMA (BBC3) and p53AIP1 (TP53AIP1) (Riley et al, 2008) or mTORC1 regulators TSC2, AMPKβ (PRKAB1 and PRKAB2) and PTEN (Feng et al, 2007), did not show differential expression between the 'p53-unknown' and 'p53-mutated' tumor groups (Figure 4—figure supplement 1D–J), suggesting that the effects of p53 mutations on these genes are minimal in the pathological context of colon carcinogenesis. This evidence concerns the gene TSC2 and neoplasm.