SESN2 and colonic neoplasm: To understand if Sestrin2, acting as a p53 target, has an essential role in regulating mTORC1 and colon cancer cell growth, Sestrin2 was overexpressed in p53-deficient HCT116 cells and SW480 cells, which have low SESN2 expression and high mTORC1 signaling (Figure 7A,B).