In this study, we demonstrated that miR-193a-3p and miR-193a-5p suppress the metastasis of human osteosarcoma cells by repressing Rab27B and SRR expression, through suppressing the TGFβ, Myc/Max and ATF2/ATF3/ATF4 signaling pathways. This evidence concerns the gene ATF4 and osteosarcoma.