In this study, the multivariable logistic regression showed that only age and T lymphocytes CD8+ CD28-CD57+ were independently associated with the presence of two comorbidities; in particular, the ROC curve revealed that a cut-off>8.87% of T lymphocytes CD8+ CD28-CD57+ correlated with the presence of concomitant bone and cardiovascular disease, suggesting that the immune exhaustion during HIV infection might exert a prominent role in the development of these conditions. This evidence concerns the gene CD28 and HIV infectious disease.