IL1B and infection: The subcutaneous injection of a high dose of L. major (4 × 106 promastigotes/50 μl nutrient broth medium/left hind paw) caused a significant change in the levels of IL-1 in the infected paws of mice starting day 2 post-infection when it increased from 2.75 ± 0.67 pg/hind paw in the non-infected mice to 146 ± 7.32 pg/hind paw (p < 0.001).