The subcutaneous injection of a high dose of L. major (4 × 106 promastigotes/50 μl nutrient broth medium/left hind paw) caused a significant increase in the level of KC at day 2 post-infection in the infected hind paw of the mice (104 ± 2.28 pg/hind paw) (p < 0.001) as compared to the level in the control group (5.83 ± 0.124 pg/hind paw). This evidence concerns the gene CALCA and infection.