In the present study, we analyzed the expression of MIF in human breast cancer cell line which was knocked down the MIF messenger RNA (mRNA) using small interfering RNA and expression changes of VEGF-C, p38-MAPK, P-p38MAPK, p44/42-MAPK, and P-p44/42-MAPK to discuss the effect of MIF on VEGF-C and the involved possible molecular mechanisms. This evidence concerns the gene VEGFC and breast carcinoma.