Our data showed miR-410 played a reverse role in regulating the expression of DVL2, β-catenin and Gsk3β compared with SLC34A2. And up-regulation of DVL2 and β-catenin and down-regulation of Gsk3β by miR-410 were partly rescued by SLC34A2. It indicated that miR-410 activated Wnt/β-catenin signaling pathway via down-regulating SLC34A2 in NSCLC. This evidence concerns the gene GSK3B and non-small cell lung carcinoma.