In addition, we found that RUNX1high was associated with FLT3-ITD, non double CEBPA mutation and higher ERG, WT1, DNMT3B, TCF4, MIR155HG, ITPR2, MAPKBP1 expression, all of which are unfavorable molecular characteristics in CN-AML patients. Here, TCF4 is linked to acute myeloid leukemia.