Conversely, strong immunostaining for TGF-β1, AEG-1, MAP-LC3, N-cadherin is observed in the glioblastoma multiforme (grade IV) and also in astrocytoma (grade II), oligodendroglioma and oligoastrocytoma (grade III). The gene discussed is CDH2; the disease is astrocytoma (excluding glioblastoma).