Finally, the observation that the rate of MET ectodomain shedding – in terms of the number of N-terminal fragments – increases with increasing malignant behavior of breast cancer cells [38], provides an explanation for the p70MET and p60MET C-terminal fragments observed in HeLa, HT-29 and PC3 using D1C2 under reducing conditions. This evidence concerns the gene MET and breast carcinoma.