Furthermore, insulin vials were checked weekly to assess treatment adherence, and all patients systematically received instructions for both insulin administration and glucose monitoring by the nutritionist involved in the study, who received training in Diabetes Education by the Brazilian Society of Diabetes (Sociedade Brasileira de Diabetes – SBD) and the Juvenile Diabetes Association (Associação de Diabetes Juvenil – ADJ). This evidence concerns the gene INS and diabetes mellitus.