Consistently, Akt phosphorylation was reduced in eWAT and iWAT from HFD-fed A-Nicastrin mice (Figure 5I and not shown), which also showed a relative hyperinsulinemia and excess circulating fatty acids (Figure 5J–L) resulting in a trend towards increased liver weight and triglyceride content (Supplementary Figure. This evidence concerns the gene AKT1 and hyperinsulinism.