ANXA5 and head and neck squamous cell carcinoma: To determine whether FHL1 expression induced apoptosis in HNSCC cells, we assessed the fraction with positive staining for 7-amino-actinomycin (7-AAD) and Annexin V-PE in HNSCC cells with FHL1 overexpression and the control cells (47.81% vs 26.28% for HN-13 and 13.91% vs 12.08% for CAL-27; Figure 4A), suggesting that apoptosis-induced inhibitory effect processes a cell-specific manner.