To further confirm the role of Sp1 and NF-κB in the induction of Inpp5f under diabetes context, mouse Inpp5f regulatory region (−734 ~ +1, with the A in ATG designated as +1), which covers about 500 bp promoter region and part of the first exon, was cloned into pGL3 basic reporter vector. Here, INPP5F is linked to diabetes mellitus.