MCAM and melanoma: When compared to the positive control cell line, human melanoma SK-Mel-28 cells (assuming expression of 100 % of METCAM/MUC18) (lane 1), the METCAM clone 2D-9 (lane 3) and the METCAM clone 2D (lane 4) of SKOV3 cells showed much higher expression of METCAM/MUC18 (137 and 51 %, respectively) than that of clone of the control (vector) clone 3D (lane 5), which expressed 0 % of METCAM/MUC18, similar to the parental SK-OV-3 cells (lane 2).