To characterize the relationship between FGFR1 gene amplification and expression in sqNSCLC tissues, a panel of 90 tumour samples was profiled for FGFR1 amplifications by FISH analysis using a specific FGFR1 gene probe (RP11-957P17 Chr8: 38255823–38443297 (GRCh37/hg19)). Here, FGFR1 is linked to neoplasm.