So various assays were adopted to evaluate the effects of GA on the activation of NF-κB. As assessed by western blot and shown in Figure 6(a), we found that the expression of IκBα was decreased, while the expressions of p-NF-κB and NF-κB in nucleus were increased significantly in sepsis group, compared with sham group, which demonstrated the activation of NF-κB induced by AKI. The gene discussed is NFKB1; the disease is acute kidney injury.