The confocal fluorescence microscopy analysis of YAP and α-SMA double staining showed that the expression of YAP was reduced in VSMCs from the aortic walls of patients with ascending aortic aneurysms (Figure 2(d)), which revealed the downregulation of YAP in VSMCs of ascending aortic aneurysms. This evidence concerns the gene ACTA1 and Ascending tubular aorta aneurysm.