Our result also demonstrated that in human glioma brain cell lines Pygo2 plays a crucial role in activating the transcription of Wnt genes and regulating the recruitment of f WDR5 and Ash2, two core components of the COMPASS complex, and menin, a specific component of the MLL1/MLL2 histone methyltransferase (HMT) complex, to the Wnt pathway target gene promoter loci. The gene discussed is PRDM9; the disease is central nervous system cancer.