We also used western blot and qRT-PCR analyses to examine the expression of Pygo2 and β-actin (as control) in five glioma cell lines, TJ861, A172, U373, U251 and U-87MG and found that Pygo2 protein and mRNA levels are both up-regulated as compared with normal human microglia primary cells (Glia) (Fig. 1F–H). This evidence concerns the gene PYGO2 and glioma.