In contrast, with the increase of glioma malignant potential, the nuclei of astrocytoma tissues were round, triangle or fusiform, and cells were moderately abnormal, with uneven distribution and a single or dual core, Pygo2 protein was mainly expressed in the nucleus and much less in the cytoplasm (Fig. 3B, “+”). Here, PYGO2 is linked to astrocytoma (excluding glioblastoma).