ASCL1 and neuroblastoma: At molecular level, ATRA-induced SK-N-BE(2)C cells differentiation is characterised by changes in the expression of some target genes, including HASH1[40], [41], and Northern blot analysis of RNA from the cells treated with ATRA for different times revealed a unique transcript of approximately 3 Kb encoding HASH1 (Fig. S1B, lanes 1, 2 and 4), which was observed in all of the tested NB cells, whereas no signal was observed in the HeLa cells (Fig. S1B, lane 3).