Next, western blots for BRCA1 and AR were performed on extracts of breast cancer cell lines representing different molecular subtypes, namely MCF-7 and T47D (Luminal type, ER+/PR+/HER2−), SK-BR-3 (HER2 enriched, ER−/PR−/HER2+), and MDA-MB-157 (basal type, ER−/PR−/HER2−) transfected with BRCA1 clones. The gene discussed is BRCA1; the disease is breast cancer.