After adjustment for age, sex, race, smoking status, alcohol intake, education degree, physical activity, family income, BMI, and history of CVD, we found that both elevated ALT and elevated AST were associated with higher prevalence of MetS (OR 3.020, 95% CI: 2.496–3.653 for elevated ALT; OR 1.689, 95% CI: 1.314–2.171 for elevated AST). This evidence concerns the gene GPT and metabolic syndrome.