34, 35 We first examined C-peptide by dividing 44 T1D subjects by presence or absence of C-peptide and looked at the association with the presence or absence of aTregs (absence of aTregs was defined as aTregs/CD4 cells <1.5%, while presence of aTregs was defined as >1.5%). Here, CD4 is linked to type 1 diabetes mellitus.