The signaling pathways up-regulated in LPS-treated JS1 cells included Toll-like receptor, neurotrophin signaling, glycolysis/gluconeogenesis, and immune disease pathways (Table 3), with MAPKs (MAPK9, MAPK14) and multiple MHC molecule (H2-Q2); these were the core regulatory factors in gene-act-net work (Additional file 3: Figure S2) and co-expression network (Additional file 4: Figure S3), with the highest degree and differential degree numbers. This evidence concerns the gene BDNF and immune system disorder.