FOXO1 and Insulin resistance: Decreasing Akt activity by incubating MIN6 cells with the pharmacological inhibitor Akti-1/2 (10 μM) and thus mimicking insulin resistance led to an increase in the nuclear localization of FoxO1(H215R)GFP (green signal), while the reference Tomato (red signal) remains cytosolic (Fig. 2a,b).