RNA expression analyses highlighted the upregulation of glycolytic genes typically associated with metabolic alterations in GTICs in the transformed iNPCs, including: PKM2; HK; GLUT3 (a gene recently described as critical for glucose uptake in GTICs)6; and LDHB (a gene commonly upregulated in gliomas bearing IDH mutations)32 (Supplementary Fig. 3a). This evidence concerns the gene PKM and central nervous system cancer.