Furthermore, serial transplantation of Ras/EGFR/SrciNPCs and p53KD-Ras/EGFR/SrciNPCs resulted in the formation of highly aggressive and infiltrative secondary tumours, presenting similar histological characteristics to those observed in the respective primary tumours, even upon secondary transplantation of 500 cells (Supplementary Fig. 6b–e and Supplementary Table 3). The gene discussed is EGFR; the disease is neoplasm.