TLR4 was immunolocalized in vimentin-positive and CK19-negative stromal cells, MD2, MyD88, NF-κB, VEGF, EGF, MMP2, IL-6 and TGF-β in CESC, EuESC and EESC were detected by IHC. This evidence concerns the gene LY96 and cervical squamous cell carcinoma.