In the present study, we first investigated the changes in serum iron and three main iron-handling proteins ferritin, Tf and CP in 100 consecutive patients with acute spontaneous ICH during the period from admission (day 1) to day 21, and then analyzed effects of intraventricular hemorrhage, hematoma volumes and surgery treatment on the contents of iron, ferritin, Tf and CP in the serum, and finally conducted correlation analysis between relative edema volume, ratio or hematoma volume and serum iron, ferritin, Tf or CP contents, and serum iron and one of three main iron-handling proteins. The gene discussed is PROS1; the disease is Intraventricular hemorrhage.