BCR and chronic myelogenous leukemia, BCR-ABL1 positive: Using the same CRISPR/Cas9 approach, we generated HEK293 cells harboring the human chronic myelogenous leukemia (CML)-associated Philadelphia chromosome by inducing chromosomal translocation between chromosomes 9 and 22, which resulted in the creation of the BCR-ABL1 fusion gene (Supplementary Fig. 4a)23.