The siRNAs against mTOR, Raptor and Rictor were employed to reduce the expression of both mTORC1 (mTOR and Raptor) and mTORC2 (mTOR and Rictor) in Cal27 cells, an established cell line derived from the poorly differentiated squamous cell carcinoma of the tongue [35]. Here, MTOR is linked to squamous cell carcinoma.