No effect was seen after treating cells with PI3K isoform-selective inhibitors (TGX221, AS605240 and IC87114 for p110β, p110γ and p110δ, respectively) at doses known to discriminate different isoforms, whereas, MDA-MB231 growth in Matrigel was partly inhibited by the pan-PI3K inhibitor, GDC0941, or pan-AKT inhibitor, MK2206, suggesting several isoforms of PI3K and AKT work in concert to drive breast cancer cell growth (Figure 7A). The gene discussed is AKT1; the disease is breast cancer.