In order to determine the specific contribution of AMPK signaling to cell cycle progression and death during glucose deprivation in liver cancer cells, we reduced the expression of the most abundant isoform of the catalytic subunit of AMPK, AMPKα1 [18, 19], by RNA interference in HepG2/C3A, HuH-7, and SK-Hep-1. Here, PRKAA1 is linked to liver cancer.