To verify this hypothesis, we established HULC-silenced U87MG and U251 cell lines, and the results showed that (1) HULC silencing suppresses ESM-1-mediated proliferation, adhesion, migration, invasion and angiogenesis through the cell cycle and anoikis regulation in the glioma U87MG and U251 cell lines and (2) HULC is an oncogene in the PI3K/Akt/mTOR signaling pathway and up-regulates the expression of ESM-1. Here, HULC is linked to glioma.