However, there was also some question, for example, (1) whether VEGF generate the same results as ESM-1 on HULC silenced cells; (2) whether the effects of ESM-1 be blocked by its antibody such as LFA1; (3) whether there was another effect of hypoxic mimicry with cobalt chloride on glioma biological behavior and what's the effect of HULC silencing on it, which need to further explored. The gene discussed is VEGFA; the disease is glioma.