To explore the mechanism underlying the regulation of glioma angiogenesis by HULC, we silenced HULC in U87MG and U251 cells and found that HULC silencing reversed the potential for glioma angiogenesis and influenced key processes such as proliferation (including anchorage-independent proliferation), migration, adhesion, invasion, the cell cycle (arrested in G1/S phase) and anoikis in vitro. This evidence concerns the gene HULC and central nervous system cancer.