PDPN and melanoma: All EV preparations that were isolated by standard procedures (crude EXO fractions; see Materials and methods) from different cell lines: HN5 cells and MDCK or human melanoma SK-MEL-28 cells expressing PDPN tagged with enhanced green fluorescent protein (MDCK-PDPNeGFP, SK-MEL-28-PDPNeGFP), contained PDPN when analyzed by Western blotting (Figure 1).