To determine the mechanistic aspects of how mDia1 controls MT1-MMP to induce breast cancer invasion, we first examined whether knockdown or overexpression of mDia1 regulates MT1-MMP expression, since it was reported that silencing of mDia1 also suppressed the expression of myogenic regulatory factor proteins such as MyoD [22]. This evidence concerns the gene MYOD1 and breast carcinoma.