To determine whether the FoxO1 protein participates in ZBTB20-mediated cell proliferation and cell-cycle progression in HCC, FoxO1 was subsequently up-regulated in ZBTB20 overexpressing SMMC-7721 cells via plasmid transfection (P < 0.01, Figure 6A). The gene discussed is FOXO1; the disease is hepatocellular carcinoma.