KCNB1 and Alzheimer disease: To determine whether the reduction in macroscopic currents that we have observed in 3xTg-AD cultures is linked to increased ROS production and subsequent ROS-dependent loss of function of Kv2.1 channels, we examined cytosolic ROS levels evoked by a mild excitotoxic challenge obtained with exposure to N-methyl-d-aspartate (NMDA).