HSPA5 and hepatocellular carcinoma: Treatment of HuH7 hepatoma or HT1080 sarcoma cells with OSU-03012 +/− sildenafil or sorafenib +/− sildenafil resulted in a rapid dose- and time-dependent reduction in the detection of the ATP binding and ATPase competent chaperone proteins HSP90, GRP78 and HSP70 in fixed in situ cells as judged using immuno-fluorescence with 0.5% (v/v) Triton-X100 for cell permeabilization from a Hermes WiScan wide field microscope (Figure 1A and 1B).