We further investigated BMI-1 transcript expression in primary blast cells from Ph+ and Ph− ALL patients after in vitro treatment with Nutlin-3a observing a trend of reduction in Ph+ ALL cells if compared with their untreated counterparts (Figure 6B); instead, Ph− ALL samples showed a different expression of BMI-1 after treatment (Figure 6C). This evidence concerns the gene BMI1 and acute lymphoblastic leukemia.