To further study the role of ANT1 in FA-induced uncoupling in relation to insulin responsiveness, we also examined isolated skeletal muscle mitochondria obtained from a rodent model of type 2 diabetes (Zucker diabetic fatty [ZDF] rats) and employed a small interfering RNA (siRNA)-mediated gene silencing of Ant1 in C2C12 myotubes. This evidence concerns the gene INS and type 2 diabetes mellitus.