IFNG and melanoma: For the NY-ESO-196–104 epitope, incubation of melanoma cell lines with PR-924 alone had generally little effect on T-lymphocyte recognition or cytotoxicity, however co-incubation with PR-924 and IFNγ resulted in decreased T-lymphocyte responses compared to IFNγ alone, which in most cases reverted to levels comparable with standard proteasome mediated processing of this epitope (Fig. 5b, e).