For the NY-ESO-192–100 epitope, T-lymphocyte activation following exposure to melanoma cells treated with IFNγ in conjunction with PSMB8 knock-down, was substantially decreased in comparison to IFNγ together with control siRNA, and indeed was reduced to below that of untreated control cells (Fig. 4c), most likely reflective of knockdown of low level PSMB8 expression in these cell lines under steady state conditions (Fig. 1). The gene discussed is PSMB8; the disease is melanoma.