Lymphodepletion with 5Gy TBI enhances a tripartite ACT treatment consisting of PFI (P = infusion of 1e6 pmel-1 CD8+ T cells, F = vaccination with fowlpox encoding hgp100 and I = high dose IL-2) in mice with B16F10 melanoma to a greater extent than in lymphoreplete mice (Fig. 1a). This evidence concerns the gene IL2 and melanoma.