Overall, this study has investigated methylation of the Wnt genes APC and ITF2 in a large cohort of MSI-H and matched MSS CRC tumors to find that ITF2 methylation is significantly associated with MSI-H tumors while APC methylation is a tumor-specific event in CRC, which does not differ significantly between MSI-H and MSS subtypes or other clinicopathological variables. Here, TCF4 is linked to colorectal carcinoma.